Betekenis van:
inoculum

Voorbeeldzinnen

1. Inoculum
2. Preparation of inoculum culture
3. Three examples of suitable inoculum are given:
4. A further source for the inoculum is surface water.
5. The inoculum culture shall be incubated under the same conditions as the test cultures.
6. Measure of the dosage (inoculum size) required to cause a specific degree of pathogenicity.
7. If no haemagglutination is detected, this procedure must be repeated using undiluted allantoic/amniotic fluid as inoculum.
8. To avoid synchronous cell divisions during the test, a second propagation step of the inoculum culture may be required.
9. Any practice resulting in the removal, destruction or burial of infected crop residues, such as ploughing, is likely to reduce the Fusarium inoculum for the following crop.
10. Measure the increase in biomass in the inoculum culture to ensure that growth is within the normal range for the test strain under the culturing conditions.
11. If the equipment used for biomass measurement allows sufficiently precise determination of the low inoculum biomass (e.g. flow cytometer) then the measured initial biomass concentration can be used.
12. The algal biomass should be adjusted in order to allow exponential growth to prevail in the inoculum culture until the test starts.
13. Colonies consisting of 2 to 4 visible fronds are transferred from the inoculum culture and randomly assigned to the test vessels under aseptic conditions.
14. Test solutions of the chosen concentrations are usually prepared by mixing a stock solution of the test substance with growth medium and inoculum culture.
15. Prepare an inoculum of approximately 106 cells per ml from three day cultures of the isolate to be tested and an appropriate positive control strain of C. m. subsp. sepedonicus.