Betekenis van:
phosphatase

phosphatase
Zelfstandig naamwoord
    • any of a group of enzymes that act as a catalyst in the hydrolysis of organic phosphates

    Hyperoniemen


    Voorbeeldzinnen

    1. Phosphatase
    2. Phosphatase activity
    3. Phosphatase test
    4. DETERMINATION OF ALKALINE PHOSPHATASE ACTIVITY
    5. Alkaline phosphatase substrate solution, pH 9,8
    6. sufficient to ensure a negative reaction to a phosphatase test,
    7. A unit of alkaline phosphatase activity is the amount of alkaline phosphatase enzyme that catalyses the transformation of 1 micromole of substrate per minute.
    8. Prepare the appropriate dilution of secondary antibody-alkaline phosphatase conjugate in blocking buffer.
    9. Prepare an appropriate dilution of anti-mouse immunoglobulins conjugated with alkaline phosphatase in PBS.
    10. The alkaline phosphatase activity is expressed as milliunits of enzyme activity per litre (mU/l).
    11. When determining alkaline phosphatase activity, ISO standard 11816-1 must be applied as reference method.
    12. Add 100 µl alkaline phosphatase substrate solution (Appendix 4) to each well.
    13. after having undergone heat treatment such as to show a negative reaction to the phosphatase test;
    14. Dissolve 2 phosphatase substrate 5 mg tablets (Sigma) per 15 ml of solution.
    15. after having undergone heat treatment such as to show a negative reaction to the alkaline phosphatase test; and