Betekenis van:
pellet

Voorbeeldzinnen

1. Pellet 1
2. Decimal dilution of resuspended pellet
3. Prepare decimal dilutions (1/10 and 1/100) of the resuspended pellet in pellet buffer.
4. Pellet buffer (10 mM phosphate buffer, pH 7,2)
5. Samples with less than 5 × 103 typical cells per ml of resuspended pellet are considered negative.
6. Process the heel ends as usual and resuspend the pellet in 10 ml.
7. Allow the pellet to dry in air or in a DNA speed vac.
8. Centrifuge at 15000 g for 20 minutes at 4 °C and remove ethanol from pellet.
9. Centrifuge at 15000 g for 10 min at 4 °C, save the pellet and remove ethanol.
10. Remove the supernatant and dissolve the pellet in 200 µl of fixative prepared < 24 hours previously.
11. Centrifuge 30 to 50 ml sub-samples at 10000 g for 10 minutes (or 7000 g for 15 minutes) preferably at 4 to 10 °C, discard the supernatant and resuspend the pellet in 1 ml pellet buffer (Appendix 4).
12. Wilts may also be induced by populations of other bacteria or fungi present in the tuber tissue pellet.
13. Subsequently pipette a similar volume of undiluted pellet (1/1) onto the remaining windows on the row.
14. To use this dissolve the pellet from step 5.1.2 in 50 µl 0,01M PB and 50 µl 96 % ethanol.
15. Output of all sinter pellet and other plants producing agglomerated materials for blast furnace charge and directly reduced sponge iron