Betekenis van:
inoculation

Voorbeeldzinnen

1. Syringe inoculation
2. Slit inoculation
3. Day after inoculation
4. Inoculation and incubation of eggs
5. Isolate from symptomatic plants by removing a section of stem 2 cm above the inoculation point.
6. Do not water eggplants for one to two days prior to inoculation to reduce turgor pressure.
7. Isolate from symptomatic plants by removing a section of stem about 2 cm above the inoculation point.
8. any non-standard operations (e.g. the inoculation of animals with GMMs; use of equipment likely to generate aerosols).
9. As the negative control, inoculate 5 plants with sterile pellet buffer by the same inoculation method (7.3 or 7.4).
10. Eggplants or tomatoes should be grown in a glasshouse with the following environmental conditions prior to inoculation:
11. In the case of the biological testing, the maximum bulking is up to five plants with inoculation of a minimum of duplicate indicator plants.
12. In order to ensure complete utilisation of the resuspended pellet as well as effective inoculation the procedures outlined below will require 15 to 25 eggplants per sample.
13. However, when testing pigs in contact with AI viruses, when virus has had little opportunity to adapt, allantoic cavity inoculation is probably sufficient.
14. Subculturing of C. m. subsp. sepedonicus-like colonies should be carried out onto YGM media for eggplant inoculation and/or subsequent identification; this should be done before the plates become too overgrown i.e. preferably after three to five days.
15. If no symptoms are observed after three weeks perform IF/PCR/Isolation on a composite sample of 1 cm stem sections of each test plant taken above the inoculation site.